Coding

Part:BBa_K4475000:Design

Designed by: Andrew Taylor   Group: iGEM22_FSU   (2022-07-17)


CTB1 Gene from Cercospora beticola


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3532
    Illegal BamHI site found at 3441
    Illegal BamHI site found at 5614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6121
    Illegal AgeI site found at 4195
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was determined from an mRNA sample of Cercospora Beticola (listed under references). The sequence was also codon optimized for yeast, silently mutated to avoid restriction enzyme cut sites and tagged with an N-terminus HA tag.

Source

https://www.ncbi.nlm.nih.gov/nuccore/1331157726

Taken From

de Jonge R, Ebert MK, Huitt-Roehl CR, Pal P, Suttle JC, Spanner RE, Neubauer JD, Jurick WM 2nd, Stott KA, Secor GA, Thomma BPHJ, Van de Peer Y, Townsend CA, Bolton MD. Gene cluster conservation provides insight into cercosporin biosynthesis and extends production to the genus Colletotrichum. Proc Natl Acad Sci U S A. 2018 Jun 12;115(24):E5459-E5466. doi: 10.1073/pnas.1712798115. Epub 2018 May 29. Erratum in: Proc Natl Acad Sci U S A. 2018 Aug 28;115(35):E8324. PMID: 29844193; PMCID: PMC6004482.